Last edited by Arashilar
Friday, July 10, 2020 | History

2 edition of Studies on defective interfering Semliki Forest virus found in the catalog.

Studies on defective interfering Semliki Forest virus

Alan D. T. Barrett

Studies on defective interfering Semliki Forest virus

by Alan D. T. Barrett

  • 94 Want to read
  • 0 Currently reading

Published by typescript in [s.l.] .
Written in English


Edition Notes

Thesis (Ph.D.)-University of Warwick, 1984.

StatementAlan D.T. Barrett.
ID Numbers
Open LibraryOL14830224M

  Preparations of defective-interfering (DI) Semliki Forest virus (SFV) differed qualitatively in their ability to protect mice against lethal SFV-induced by: Influenza A and B viruses are major human respiratory pathogens that contribute to the burden of seasonal influenza. They are both members of the family Orthomyxoviridae but do not interact genetically and are classified in different genera. Defective interfering (DI) influenza viruses have a major deletion of one or more of their eight genome segments, which renders them both non-infectious Cited by:

The Semliki Forest virus was first isolated from mosquitoes in the Semliki Forest, Uganda by the Uganda Virus Research Institute in and described by Smithburn and Haddow. It is known to cause disease in animals including humans. It is an alphavirus found in central, eastern, and southern : Togaviridae.   Defective viral genomes are key drivers of the virus–host interaction S. Studies on interference in experimental influenza. from the modulation of a lethal dose of Semliki Forest virus Cited by:

CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): Molecular and host range properties of the virulent L10 strain of Semliki Forest virus were compared to those of the avirulent A7 strain. No difference could be detected between the two strains in adsorption, nucleocapsid synthesis, protein synthesis, ratio of 42SS RNA, particle infectivity, interferon induction.   Semliki Forest virus (SFV) vectors are promising tools for cancer gene therapy because they ensure a high level of transgene expression and a Cited by: 9.


Share this book
You might also like
GCSE examination

GCSE examination

Bunkers

Bunkers

Fire Safety in the Operation of Nuclear Power Plants

Fire Safety in the Operation of Nuclear Power Plants

plays of Shakspere

plays of Shakspere

Solomons Temple

Solomons Temple

Potential hazards from future eruptions in the vicinity of Mount Shasta volcano, northern California

Potential hazards from future eruptions in the vicinity of Mount Shasta volcano, northern California

Save the Florida Manatee (Save Our Animals)

Save the Florida Manatee (Save Our Animals)

Standards

Standards

Water resources of southwestern Louisiana

Water resources of southwestern Louisiana

Catalogue of the Fiji Museum

Catalogue of the Fiji Museum

Cock-a-Doodle-Doo

Cock-a-Doodle-Doo

Legal analysis of the application of section 1886(g)(1) of the Social Security Act concerning Medicare payment for capital-related costs in view of proposed rules issued May 19, 1987

Legal analysis of the application of section 1886(g)(1) of the Social Security Act concerning Medicare payment for capital-related costs in view of proposed rules issued May 19, 1987

Fertilizer manufacturing projects

Fertilizer manufacturing projects

principles of education for African teachers in training.

principles of education for African teachers in training.

The statutes at large

The statutes at large

Studies on defective interfering Semliki Forest virus by Alan D. T. Barrett Download PDF EPUB FB2

A) History Semliki Forest virus (SFV) was originally isolated from a pool of female Aedes abnormal is mosquitoes captured in Bundinyama, Uganda in (Smithburn and Haddow, ).

b) Classification SFV is classified as a member of the genus alphavirus, of the family Togaviridae (Matthews, ). It was originally described as an arbovirus ("arthropod-borne" virus) (Casals and Brown, Author: Alan D. Barrett. Oligonucleotide mapping studies of the RNA from standard and defective interfering particles of Sindbis virus demonstrate that 3'- and 5'-terminal regions of the genome are conserved in the.

Studies of defective interfering RNAs of Sindbis virus with and without tRNAAsp sequences at their 5' by: Defective interfering Semliki Forest virus populations are biologically and physically heterogeneous output: Contribution to journal › Article.

9 Scopus citations. Abstract. This study demonstrates that populations of defective interfering Semliki Forest virus (DI SFV) are heterogeneous particularly in respect of their interference Cited by: CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): Purified defective-interfering (DI) particles of Semliki Forest virus are unable to carry out any of the steps in virus multiplication except uncoating.

Cells co-infected with DI particles and standard virus contain several virus-specified RNA species (DI particle-specific species) absent from cells infected with. The majority of mice inoculated with a mixture of a lethal dose of virulent Semliki Forest virus (SFV) strain ts' and defective-interfering (DI) SFV remained completely healthy, with virus infectivity levels in brain tissue reduced by %/o.

The results of previous studies had suggested that these effects were primarily the result of the. Abstract. The majority of mice inoculated with a mixture of a lethal dose of virulent Semliki Forest virus (SFV) strain ts: and defective-interfering (DI) SFV remained completely healthy, with virus infectivity levels in brain tissue reduced by Cited by:   Serial passaging of Semlike Forest virus in BHK cells at a constant input multiplicity of 50 p.f.u./cell resulted in a 4 log10 drop in yield of infectious virus by passage 9.

An interference analysis showed that this drop was due to the presence of defective-interfering (DI) by: cloned cDNA copy of an 18S defective interfering (DI) RNA of Semliki Forest virus has been determined. This corresponded to a major virus-specific cytoplasmic RNA species at the 11th undi-luted passage of the virus in BHK cells.

The nucleotide-long sequence consists of a unique 5'-terminal sequence followed by. In the past few years, many studies have been done on the structure of DI particles and the mechanisms of interference by and generation of these particles [1, 5, 6]; most studies have centered on vesicular stomatitis virus [7], influenza virus [8], and the alphaviruses Sindbis virus and Semliki Forest virus (SFV) [9, 10].

However, little is known. The 1,nucleotide genome of Semliki Forest virus (SFV) defective interfering (DI) RNA 19 (DI) is coterminal with the infectious genome and contains two major deletions.

One deletion removes the end of the nsP1 gene and the beginning of the nsP2. The majority of mice inoculated with a mixture of a lethal dose of virulent Semliki Forest virus (SFV) strain ts + and defective-interfering (DI) SFV remained completely healthy, with virus infectivity levels in brain tissue reduced by %.

The results of previous studies had suggested that these effects were primarily the result of the intrinsic interfering capacity of DI virus rather than of Cited by: Eaton BT. Defective interfering particles of Semliki Forest virus generated in BHK cells do not interfere with viral RNA synthesis in Aedes albopictus cells.

Virology. Dec; 68 (2)– HERS JF, MUDLER J, MASUREL N, vd KUIP L, TYRRELL DA. Studies on the pathogenesis of influenza virus pneumonia in mice. J Pathol by: The 1,nucleotide genome of Semliki Forest virus (SFV) defective interfering (DI) RNA 19 (DI) is coterminal with the infectious genome and contains two major deletions.

The nucleotide sequence of a nearly full-length cloned cDNA copy of an 18S defective interfering (DI) RNA of Semliki Forest virus has been determined. This corresponded to a major virus-specific cytoplasmic RNA species at the 11th undiluted passage of the virus in BHK cells.

Assay of Defective-interfering Semliki Forest Virus by the Inhibition of Synthesis of Virus-specified RNAs Article (PDF Available) in Journal of General Virology 54(Pt 2) July with. By electron microscopy, particles of defective interfering Semliki Forest virus (DI SFV) had a mean diameter of nm compared with nm for standard virus particles, a decrease of 16%.

Pettersson RF. 5'-Terminal nucleotide sequence of Semliki forest virus 18S defective interfering RNA is heterogeneous and different from the genomic 42S RNA. Proc Natl Acad Sci U S A. Jan; 78 (1)– [PMC free article] Käriäinen L, Simons K, von Bonsdorff CH.

Studies in subviral components of Semliki Forest virus. VIROLOGY() Multiple Structurally Related Defective-Interfering RNAs Formed during Undiluted Passages of Semliki Forest Virus LEEVI KRINEN,1 RALF F. PETTERSSON, SIRKKA KEREN, PVI LEHTOVAARA, HANS SERLUND, AND PENTTI UKKONEN Department of Virology and the Recombinant DNA Laboratory, University of Helsinki, Haartmaninkatu 3, SF Helsi Cited by: Among the togaviruses, extensive studies of defective interfering (DI) particles have so far been carried out only with Sindbis virus (SV) and Semliki Forest virus (SFV), both members of the alphavirus genus.

Since these viruses are so similar, in most cases it will be assumed that what is true of one is also true of the other. Definition. Production of defective interfering virus in the brains of mice by an avirulent, in contrast with a virulent, strain of Semliki forest by: 9.CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): This study demonstrates that populations of defective interfering Semliki Forest virus (DI SFV) are heterogeneous particularly in respect of their interference properties.

Interference was quantified by two assays, one measuring inhibition of the yield of infectious progeny virus, and the other measuring reduction in.Weiss B, Schlesinger S. Defective interfering particles of Sindbis virus do not interfere with the homologous virus obtained from persistently infected BHK cells but do interfere with Semliki Forest by: